Chapter 2 describes the isolation of the composite monosaccharides of alginate (D mannuronate and L guluronate) and their characterisation by NMR spectroscopy. A combination of 1 and 2 D 1H and 13C experiments were utilised to provide the most comprehensive assignments of algal mono uronates to date. Subsequently, the well defined mono uronate spectra were used to probe the conditions that favour hydrothermal uronolactone formation.

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